The effect of growth regulators on micropropagation of medicinal plant Chrysanthemum hortorum hort by using plant tissue culture technology

Abstract

This study was conducted to evaluate the impact of various concentrations of growth regulators added to the MS on the propagation of Chrysanthemum plants outside the living body, a study was conducted in the Plant Tissue Culture Laboratory at the Department of Horticulture, College of Agriculture, University of Kerbala. The findings of study can be summed up as follows:The best treatment for sterilizing the vegetative parts  was by using a concentration of 4.5% of sodium hypochlorite (NaOCl) for 15 minutes. The concentration of the benzyl adenine gave 2 mg L-1 highest percentage in the number of shoots, length and number of sheets of 6.80 sections -1 and 4.30 cm and 25.00 leaves, respectively, comparison by the comparative processing given by Lowest rates, when grow shoot tips measurement of 1 cm on the MS media. When growing vegetative shoots from the multiplication stage on the MS nutrient media, which is equipped with various concentrations of NAA for rooting, the concentration exceeds 1 mg L-1 of it to achieve the highest percentage of rooting, the number and length of roots, the length of the shoot, and the fresh and dry weight of the shoot reached 100%, respectively, 18.50 root, 5.50 cm, 13.30 cm, 2.80 and 0.98 mg), compared with the control.