Evaluation of antibacterial efficacy of biologically synthesized silver Nanoparticles using stem barks of Boswellia ovalifoliolata Bal. and Henry and Shorea tumbuggaia Roxb

Abstract

The synthesis of metal nanoparticles using biological systems is an expanding research area due to the potential applications in nanomedicines. Nanoparticles synthesized by chemical method are not eco-friendly. The biological synthesis of silver nanoparticles is cost effective and eco-friendly to that of conventional method of nanoparticles synthesis. In the present study the silver nanoparticles synthesizes rapidly by using the stem barks of endemic medicinal plants Boswellia ovalifoliolata and Shorea tumbuggaia. After assessing the formation of silver nanoparticles with the help of UV-Visible spectroscopy and were characterized by using EDAX and SEM. Diversity has been observed in size and shape of the silver nanoparticles synthesized in two plants. These phytosynthesized silver nanoparticles were tested for their antibacterial activity. The test cultures of Proteus, Pseudomonas, Klebsiella, Bacillus and E. coli species were used. The bacterial property of silver nanoparticles was analyzed by measuring the inhibitory zone. These silver nanoparticles synthesized from bark extract of Boswellia ovalifoliolata showed moderately toxic to the E. coli and Pseudomonas species and highly toxic to Proteus species. Phytosynthesized silver nanoparticles of Shorea tumbuggaia bark extract were moderately toxic to E. coli and Bacillus species and highly toxic to Klebsiella species. The important outcome of the study will be the development of value added products from medicinal plants of India for biomedical and nanotechnology based industries. © 2011 Asian Network for Scientific Information.