Bioethanol Production from Cassava (Manihot esculenta) Peel Using Yeast Isolated from Durian (Durio zhibetinus)

Abstract

The process of ethanol production generally involves pretreatment, hydrolysis of lignocellulosic biomass to fermentable sugars followed by fermentation of such sugars to ethanol. Waste of the cassava peel (Manihot esculenta) was hydrolysed by using sulphuric acidThis research aimed to produce of bioethanol as an alternative source of fuel using cassava peels as raw materials. Yeast isolated from Durian fruit (Durio zhibetinus) was used in the experiment for fermentation and the concentration of sulphuric acid of hydrolysis process was fermented by yeast for 1; 2; 3; 4; 5; 6; 7; and 8 days. 50 ml of Sodium Hydroxide NaOH was prepared to be added at this step to adjust the pH of the slurry until 5 and the temperature was kept at 25 °C. Nine samples were prepared at different three hydrolysis times at 121°C for 30 minutes, 45 minutes and 60 ninutes. For glucose consumption and ethanol product analysis, 2 ml of the sample were taken out at every 2 days interval until 8 days. During this fermentation process, sugar consumption was measured by DNS method, while quantification of ethanol was analyzed by Gas Chromatography. The result of this study obtained that the best time of hydrolysis process was 45 minute, where the result of concentration of glucose was 11.189 %. By virtue of that, fermentation process was influenced by shaking incubator at 6 days. the optimum concentration of sulphuric acid of the hydrolysis process was 30 minute, and duration time of fermentation process by shaking incubator was 8 days, while the concentration of bioethanol for the highest of hydrolysis and fermentation process was obtained 1.63 % ethanol.