AtROP1 negatively regulates potato resistance to via NADPH oxidase-mediated accumulation of H2O2

Abstract

Small GTPases are monomeric guanine nucleotide-binding proteins. In plants, ROPs regulate plant cell polarity, plant cell differentiation and development as well as biotic and abiotic stress signaling pathways. Results: We report the subcellular localization of the AtRop1 protein at the plasma membrane in tobacco epidermal cells using GFP fusions. Additionally, transient and stable expression of a dominant negative form (DN) of the AtRop1 in potato led to HO accumulation associated with the reduced development of de Bary and smaller lesions on infected potato leaves. The expression of the gene, a NADPH oxidase homologue in potato, was analyzed by RT-PCR. Expression of this gene was maintained in DN-AtRop1 transgenic plants after infection with In transgenic potato lines, the transcript levels of salicylic acid (SA) and jasmonic acid (JA) marker genes (respectively) were analyzed. The gene was induced dramatically whereas expression of, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with. Conclusions: In conclusion, our results indicate that DN-AtROP1 affects potato resistance to This is associated with increased NADPH oxidase-mediated HO production and JA signaling.