Investigation of recombinant therapeutic genes expression in umbilical cord blood mononuclear cells transduced with three adenoviral vectors encoding neurotrophic factors GDNF, VEGF and neural cell adhesion molecule NCAM

Abstract

To overcome the effects of neurodegeneration and stimulating neuroregeneration in the spinal cord transgenic mice with model of amyotrophic lateral sclerosis were injected intraspinally with umbilical cord blood mononuclear cells (UCBMCs) transduced with adenoviral vectors encoding for vascular endothelial growth factor, glia-derived neurotrophic factor and neural adhesion molecule. Two weeks after transplantation UCBMCs were localized in the site of injection and at a distance from it, confirming the migration capacity of the cells in spinal cord parenchyma. Using triple immunofluorescence staining UCBMCs expressing therapeutic genes were found. The study results suggest the expedience of given gene-cell construct to stimulate the neuroregeneration in central nervous system after neurotrauma, ischemic stroke and in neurodegenerative diseases.