Non-radioactive in-situ hybridisation to honeybee embryos and ovaries

Abstract

To study the function and expression of genes identified in the honeybee genome sequence, techniques for assaying gene expression need to be developed. We present a robust method for preparing honeybee embryos and ovaries for in-situ hybridisation. This protocol should prove useful for researchers wishing to use the genome sequence to study honeybee biology. © INRA/DIB-AGIB/ EDP Sciences, 2005.