High-performance liquid chromatography of structural isomers with cyclodextrin-poly(vinylamine)-coated silica columns, part III: Retention mechanism study of nitrophenol derivatives

Abstract

The liquid chromatographic retention mechanism of nitrophenol isomers by isocratic elution is investigated over a range of column temperatures and mobile phase compositions using several β-cyclodextrin (β-CD) bonded stationary phases. The high-performance liquid chromatographic supports are based on silica beads coated with a polymer containing β-CD. Thermodynamic parameters for the solute transfer from the mobile to stationary phases are determined, and complex formation constant values between solute and β-CD are calculated. The thermodynamic data shows that the solute retention mechanism is dependent on the amount of bonded β-CD, and the complexation constant is dependent on temperature and mobile phase composition.