Evaluación comparativa de dos métodos para determinar la actividad de fosfolipasa a en venenos de serpientes

Abstract

Phospholipase A activity was evaluated into six snake venoms. Spectrophotometrical method adapted for our lab conditions previously and delay lipoprotein coagulation macroscopical method were compared. In both cases the phospholipase activity was registrated in the venom of Micrurus spixii, Crotalus durissus, Bothrops brazili, Lachesis muta and Bothrops atrox in the same decrease order. However the enzymatic activity only was detected in the venom of Micrurus surinamensis with the spectrophotometrical method. In addition the results related to amounth venom with each technique achieved the major sensitive of spectrophotometrical method than macroscopical technique because of 2 to 10 minor folds are required.