Equations for Chromatographic Peak Modeling and Calculation of Peak Area

Abstract

By use of the exponentially modified Gaussian (EMG) as the skewed model, empirical equations based on peak height, width, and asymmetry measurements have been developed for the accurate and precise calculation of peak area for symmetric (Gaussian) and skewed peaks. These equations are useful in determining whether or not an experimental chromatographic peak fits the Gaussian or EMG peak model. They can also be used in quantitative analyses when electronic integration is precluded by peak overlap, base-line drift, etc. or when electronic integration is unavailable. By use of the area equations, 40 liquid chromatographic peaks were tested and 90% were found to fit the Gaussian or EMG model. © 1987, American Chemical Society. All rights reserved.