Single center study of ESBL-related strains of Enterobacteriaceae collected from clinical specimens of infants with the congenital heart disease using multiplex PCR amplification

Abstract

To study ESBL phenotypes, as well as to identify individual genotypes of β-lactamases and to study their prevalence among clinical strains of Enterobacteriaceae collected from children with congenital heart diseases. Methods. Clinical strains of Enterobacteriaceae were studied in order to determine their susceptibility to antibiotics, using an automated system, before genotyping resistance determinants using multiplex PCR. Results. During the period of the study, 10.9 % of ESBL-positive isolates were found among clinical strains of Enterobacteriaceae. Most ESBL-producing strains (33.3 %) were Klebsiella pneumoniae. Most strains producing extended-spectrum β-lactamases were isolated from the respiratory tract and accounted for 83.3 %. Conclusions. An experimental study provided new data as regards the prevalence of ESBL-producing genetic resistance determinants within the Enterobacteriaceae family, and their role in the development of complications. For the first time in Ukraine, we performed genotyping Multiplex PCR-based genotyping tests of ESBLpositive isolates. The study showed diagnostic value of molecular biological methods in identifying resistance determinants of microorganisms.