Surface phenotype of Japanese adult T-cell leukemia cells characterized by monoclonal antibodies

Abstract

We studied the surface phenotype and the functional activities of leukemic cells from three patients with Japanese adult T-cell leukemia (ATL) using the panel of OK and anti-Tac monoclonal antibodies, which react with differentation antigens and define functionally distinct T-cell subsets or activated and terminally differentiated T cells. The phenotype of ATL cells were determined to be OKT1+T3+T4+T10+T5-T8-OKIa1-, although cells from two patients suppressed pokeweed mitogen (PWM) induced normal B-cell differentiation, and cells from all patients lacked helper activity in this system. In addition, after cultivation with PWM, ATL cells from all patients were reactive with anti-Tac monoclonal antibody, and cells from one patient were reactive with OKIaI. These findings suggest that ATL cells arise from peripheral mature T-cell subsets and also suggest that the transition of surface phenotype of ATL cells to functionally mature and activated T cells occurs in culture.