The therapeutic effect of Piper longum Linn. fruits in testosterone induced polycystic ovarian syndrome in female wistar rats

Abstract

The regulation of polycystic ovarian syndrome (PCOS) is intricate but often disappointing. The intend of existing study is to investigate effects hydro alcoholic extract of Piper longum L. (HEPL) fruits in testosterone persuaded Polycystic ovarian syndrome. Female wistar arts were separated into 9 groups (n=6). Normal Control received Sesame oil as a vehicle control once daily. Testosterone (2mg/kg/s.c.) was administered for 31days to induce PCOS induction in all remained group. PCOS persuaded animals were given with Metformin (300mg/kg/oral route), Clomiphene citrate, (150mg/kg/oral), HEPL groups (200, 400 and 800 mg/kg), Combination group (Piper longum 800 mg/kg and Metformin 300mg/kg) and Combination group (Piper longum 800 mg/kg and Clomiphene citrate,150mg/kg) up to 59 days using vehicle as a Distilled water. Body weights, estrus cyclicity, biochemical, hormonal and histological orders were measured. Testosterone persuaded PCOS marked by uneven estrus cyclicity, enhanced triglycerides, cholesterol, LDL glycaemia, testosterone and LH (p<0.01-0.001) but there were low HDL and FSH (p< 0.05, 0.001) levels were analyzed. To study insulin resistance, Oral glucose tolerance test was performed. Multiple cysts had been observed with PCOS rats as compared with the control group. Also, HEPL established the estrus periodicity. These biochemical, hormonal and structural alterations were abridged by Piper longum L. Moreover, Piper longum significantly decreased Cystic follicles count, testosterone and LH (p<0.001) levels but proliferated FSH (p<0.001) concentrations. The hydroalcoholic extract of fruits of Piper longum L. showed significant amelioration of Testosterone induced PCOS.