NanopoReaTA: A user-friendly tool for nanopore-seq real-Time transcriptional analysis

Abstract

Oxford Nanopore Technologies' (ONT) sequencing platform offers an excellent opportunity to perform real-Time analysis during sequencing This feature allows for early insights into experimental data and accelerates a potential decision-making process for further analysis, which can be particularly relevant in the clinical context Although some tools for the real-Time analysis of DNA-sequencing data already exist, there is currently no application available for differential transcriptome data analysis designed for scientists or physicians with limited bioinformatics knowledge Here, we introduce NanopoReaTA, a user-friendly real-Time analysis toolbox for RNA-sequencing data from ONT Sequencing results from a running or finished experiment are processed through an R Shiny-based graphical user interface with an integrated Nextflow pipeline for whole transcriptome or gene-specific analyses NanopoReaTA provides visual snapshots of a sequencing run in progress, thus enabling interactive sequencing and rapid decision making that could also be applied to clinical cases