Potential Antimicrobial Activities of Probiotic Lactobacillus Strains Isolated from Raw Milk

Abstract

Bacteriocin are highly specific antibacterial proteins which are produced by strains of specific bacteria and showed broad range of antibacterial activity against some mastitis pathogens (S. aureus, E. coli, Y. enterocolitica, S. uberis and S. xylosus). Bacteriocin producing Lactobacillus species were isolated from raw cow, buffalo and, goat milk samples focusing Lactobacillus sp isolated from raw cow milk focusing on their safety, antimicrobial properties and further was purified by gel filtration (Sephadex G-100 column). The molecular weight of the purified bacteriocin was varied between 3-30 kDa, showed high thermal stability (up to 100°C) and was active over wide range of pH (3 to 10). The study revealed the possibility of using bacteriocin as producing lactobacilli antimicrobial agent to treatment some mastitis pathogens.