Anti-Osteoclastogenesis effects of gambir extract gel in periodontitis

Abstract

Objective: This study aimed to analyze the anti-osteoclastogenesis effect of gambir extract gel through RANKL and OPG serum levels, the number of osteoblasts and osteoclasts in alveolar bone tissue of Wistar rat. Material and Methods: Twenty-four male Wistar rats were divided into four equal groups, each receiving a different treatment: Group I received a placebo gel, Group II received Tetracycline gel, Group III received 8% gambir extract gel, and Group IV received 10% gambir extract gel. After 21 days, the serum levels of RANKL and OPG were assessed using ELISA. Hematoxylin-eosin staining of the bone tissue preparation was followed by microscopic counting of osteoblasts and osteoclasts. tial chamfer margin. The crown was made with direct composite resin materials with the aid of silicone key to create palatal enamel wall and then proceed with bilaminar technique until the crown was finished. enamel wall and then proceed with bilaminar technique until the crown was finished. Results: Significant differences in RANKL serum levels were seen in Group I (0.70 + 0.020) with Group III (0.031 + 0.014) (p=0.018) and Group I with Group IV (0.038 + 0.012) (p=0.041). Serum levels of OPG differed significantly between Groups I (0.165+0,125) and IV (0.536+0.182) p=(0.015). The number of Osteoblast in Group I (1.16+ 0.40) and IV (1.33+1.03) were significantly different (p=0.023). The number of Osteoclast in Group I (4.33+1.50) and IV (1.5+0.83) were significantly different (p=0.008). Conclusion: Gambir Extract Gel 10% inhibits osteoclastogenesis by increasing osteoblast and decreasing osteoclast numbers by more than 8%, decreasing RANKL, and elevating OPG serum levels.