Comparison of polyclonal rabbit antisera with monoclonal antibodies for serological typing of Pseudomonas aeruginosa

Abstract

A panel of 219 distinct strains of Pseudomonas aeruginosa were serotypes with a set of monoclonal antibodies prepared against the serotype strains of the Homma scheme (J.Y. Homma, Jpn. J. Exp. Med. 46:329-336, 1976). A total of 87.6% were typable, and there was very good agreement with the corresponding polyclonal serotype. A high proportion of strains that were polyagglutinating or nontypable with polyclonal antisera were agglutinated by antibody towards Homma group M.