Effect of Nanoparticles of Natural Minerals, Iron and Mangan Compounds, on The Growth and Superoxide Dismutase Activity of Bacillus Subtilis Imv B-7023

Abstract

The cultivation of Bacillus subtilis ІМV B-7023 in Spizizen medium, containing 1 g/L of bentonite or saponite stimulated the growth of these bacteria. The addition of 0.1–1 mM of Mnx2+ions to this medium resulted in increasing the number of bacteria. The cultivation of bacilli in the medium with similar concentrations of ferrum ions without NP`s had insignificant impact on their growth. However, the addition of 1 g/L of saponite to this medium led to considerable biomass increase, whereas bentonite had almost no impact on this value. The cultivation of B. subtilis bacteria in the medium, containing Mnx2+ions, resulted in nigligible increase in superoxide dismutase (SOD) activity. The addition of saponite and especially bentonite led to the considerable stimulation of SOD activity. The introduction of ferrum ions to the medium stimulated SOD activity of this strain, which maximum values amounted at the concentration of 1.5 mM Fex2+.