Cloning of an Arabidopsis cDNA encoding a chloroplast localizing sulphate transporter isoform

Abstract

A new member of the sulphate transporter gene in Arabidopsis encoding a chloroplast-localizing isoform, AST82 (accession no. AB008782) has been identified. AST82 had a sequence similarity with a cyanobacterial genome sequence, slr1776, which encodes a putative eukaryotic proton/sulphate co-transporter. Fusion proteins of AST82 and green fluorescent protein (GFP) transiently expressed in Arabidopsis leaves localized in chloroplasts, indicating that AST82 encodes a chloroplastic isoform.