AN INTRODUCTION TO DNA-RECOMBINANT TECHNOLOGY AND THEIR APPLICATION IN HUMAN THERAPUTICS

Abstract

Recombinant DNA-technology is one further step of genetic engineering, which enable us to mass production of safe, pure and effective biological products. Recombinant DNA is artificially created from two or more DNA incorporated into a single molecule. Genetic engineering, recombinant DNA technology, genetic modification/manipulation and gene splicing are terms that are applied to the direct manipulation of an organism’s gene. Recombinant DNA are used to produce biochemical’s such as hormones of therapeutic interest, haemopoietic growth factors, blood coagulation products, thrombolytic agents, anticoagulants, interferon, interleukins and therapeutic enzymes.  The biochemically derived biochemical is large extracellular proteins for use in either chronic replacement therapies or for the treatment of life threatening indications. This article focuses on basic concepts behind the recombinant DNA technique and current trend in recombinant DNA technology. Key Words: Recombinant DNA-technology, genetic engineering, thrombolytic agents, anticoagulants, interferon, interleukins.