A rapid chemical method for lysing Arabidopsis cells for protein analysis

Citations of this article
Mendeley users who have this article in their library.

This article is free to access.


Background: Protein extraction is a frequent procedure in biological research. For preparation of plant cell extracts, plant materials usually have to be ground and homogenized to physically break the robust cell wall, but this step is laborious and time-consuming when a large number of samples are handled at once.Results: We developed a chemical method for lysing Arabidopsis cells without grinding. In this method, plants are boiled for just 10 minutes in a solution containing a Ca2+chelator and detergent. Cell extracts prepared by this method were suitable for SDS-PAGE and immunoblot analysis. This method was also applicable to genomic DNA extraction for PCR analysis. Our method was applied to many other plant species, and worked well for some of them.Conclusions: Our method is rapid and economical, and allows many samples to be prepared simultaneously for protein analysis. Our method is useful not only for Arabidopsis research but also research on certain other species. © 2011 Tsugama et al; licensee BioMed Central Ltd.




Tsugama, D., Liu, S., & Takano, T. (2011). A rapid chemical method for lysing Arabidopsis cells for protein analysis. Plant Methods, 7(1). https://doi.org/10.1186/1746-4811-7-22

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free