Capillary electrophoresis of adenosine phosphates using boron-doped diamond electrodes

Abstract

A capillary electrophoresis coupled with electrochemical detection using boron-doped diamond electrode was developed for simultaneous detection of adenosine phosphates, i.e. adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP). In phosphate buffer solution pH 7, these three adenosine phosphates have similar oxidation potentials at around +0.9 V (vs. Ag/AgCl), which indicated that the oxidation occurred at the same moiety. Capillary electrophoresis, which was then performed using fused silica capillary (dia. 0.05 mm) at an applied potential of 10 KV can separate ATP, ADP and AMP with the retention times of 848 s, 1202 s, and 1439 s, respectively. Linear calibration curves with the limits of detection of 0.59 μM, 0.56 μM and 1.78 μM, respectively, can be achieved, suggested that capillary electrophoresis with electrochemical detector is promising for simultaneous detection of adenosine phosphates.