Sensitive aflatoxin b1 determination using a magnetic particles-based enzyme-linked immunosorbent assay

Abstract

A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELlSA) has been developed as new an alternative immunoassay for Anatoxin Bl determination. The method is based on conventional competitive ELISA whereby the anti-Anatoxin Bl antibody is immobilized on the magnetic particles' surface. The influence of the antibody type as well as antibody immobilization on the magnetic beads surface was investigated in detail. Also, optimum values for the general parameters of the method (e.g. tracer concentration, type of antibody, and incubation time) were established. Finally, a sensitive immunoassay method (mp-ELISA) was performed for Anatoxin B1 determination at ppt level (LOD = 1 ppt Anatoxin B1).