In-vitro activity of essential oils extracted from thymus vulgaris and origanum vulgare against candida albicans

Abstract

This study aimed to assess the antifungal efficacy of essential oils extracted from Origanum vulgare and Thymus vulgaris against C. albicans isolated from oral candidiasis patients in-vitro. Basra General Hospital was diagnosed based on the cultural and microbiological phenotypes and using Vitek2 Technique to the identification. The study including a comparison of the inhibitory efficacy resulting from the use of EOs extracted from O. vulgare and T. vulgaris versus C. albicans was performed using the Agar well diffusion method of, used seven concentrations of each essential oil: (crude,1.5,1, 0.8,0.5, 0.2,0.1) mg/mL, as well as estimation of minimal inhibitory concentration (MIC) which is 0.2 mg/mL for O. vulgare and 0.1 mg/mL for T. vulgaris. The highest effect was to O. vulgare oil crude extract, which reached 3.26 cm, while the inhibtion zone of crude T. vulgaris oil was 2.53 cm which is highly significant (p < 0.05). On the other side the study aimed to evaluate the activity of T. vulgaris oil store for one year comparing with freshly extracted oil found that the fresh oil has more activity reached to 2.53 cm and the activity of old oil reached to 1.51 cm was significant (p < 0.05), also using two typs of antifungal nystatin and fluconazole to compare with the activity of O. vulgare oil found that no effect of fluconazole and the activity of nystatin reach to 2.5 cm wich is less than the activity of O. vulgare oil wich reached to 3.26 cm comparing with the activity of antifungal highly significant p-value reached to 0.005, observed in-vitro suggests its administration may represent an alternative treatment for candidiasis. The antifungal sensitivity occurred to C. albicans, which record against fluconazole, voriconazole, caspofungin, micafungin, amphotericin B, and Flucytosine. also The study including investigation of the chemical compounds of (EOs) of O. vulgare using the chromatographic analysis(GC-MS) revealed that the main compounds present in the O. vulgare essential oil wich is the best activity againest C.albicans, revealed that the main compounds present in the essential oil were methyl-5-(1-methylethyl; 1-Pentacontanol; 2-Hexyl-1-octanol; sulfurous acid, ester; oxalic acid, 6-ethyloct-3-yl isohexyl ester 2-Hydroxy-p-cymene, and 2-Methyl-5-isopropylphenol.