Abstract
A simplified hemi-nested reverse transcriptase polymerase chain reaction (hnRT-PCR) has been developed to determine specifically the European Bat Lyssavirus 1 (EBLV-1) nucleoprotein gene. The specificity of this method was determined by using the seven genotypes of lyssavirus by RT-PCR, Southern blot and sequence analysis. Compared to the rabies diagnostic methods, the hnRT-PCR showed a higher sensitivity for the detection of small amounts of EBLV-1 virus. In view of these results, we suggest this new hnRT-PCR should be performed for the epidemiological survey of bat colonies, also providing rapid detection and genotyping of EBLV-1 until now encountered in all naturally infected bats in France. © 2003 Elsevier Ltd. All rights reserved.
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Picard-Meyer, E., Bruyère, V., Barrat, J., Tissot, E., Barrat, M. J., & Cliquet, F. (2004). Development of a hemi-nested RT-PCR method for the specific determination of European Bat Lyssavirus 1: Comparison with other rabies diagnostic methods. Vaccine, 22(15–16), 1921–1929. https://doi.org/10.1016/j.vaccine.2003.11.015
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