Molecular identification of the agent of Q fever - Coxiella burnetii - In domestic animals in State of Rio de Janeiro, Brazil

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Abstract

Introduction: Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. Methods: Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results: Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). Conclusions: The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

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Mares-Guia, M. A. M. de M., Rozental, T., Guterres, A., Gomes, R., de Almeida, D. N., Moreira, N. S., … de Lemos, E. R. S. (2014). Molecular identification of the agent of Q fever - Coxiella burnetii - In domestic animals in State of Rio de Janeiro, Brazil. Revista Da Sociedade Brasileira de Medicina Tropical, 47(2), 231–234. https://doi.org/10.1590/0037-8682-0076-2013

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