Mitochondrial DNA variation and genetic structure in old-world populations of Drosophila subobscura

Abstract

To discover the relation between mitochondrial DNA (mtDNA) polymorphism and the geographic population structure of Drosophila subobscura previously established for other genetic traits, a wide Paleartic survey was carried out. A total of 24 nucleomorphs was observed among 261 isofemale lines assayed by 11 restriction endonucleases with 38 different sites in the mtDNA cleavage map. The differentiation of the Canary Islands populations (δ̄ = 0.0119) compared with the mean among all the other continental and insular populations (δ̄ = 0.0002) is striking. Both the great divergence among Canary Islands nucleomorphs (δ = 0.021) compared with the maximum nucleomorph distance in all other populations (δ = 0.017) and the abundance of endemic nucleomorphs (11) on the Canary Islands (50% of the total number of different nucleomorphs found in the entire distribution area) suggest that this molecular differentiation most probably results from the very old age of the Canary Islands populations rather than from drift and founder effects.