Modified electrochemical methods for the determination of eugenol in clove (Syzygium aromaticum) and pharmaceutical formulations

Abstract

DCP and DPP polorographic methods have been developed for the quantitative as well as qualitative analysis of eugenol in plant products (i.e. Syzygium aromaticum) and pharmaceutical formulation (Dentogum™). Eugenol produces a well defined polarographic wave/peak in 0.1 M ammonium tartrate at pH 6.7 ± 0.1 with E1/2 Ep. = -1.03/ -1.20 V vs SCE. The wave/ peak height is found to be proportional to the concentration of eugenol. The developed procedure was used for the analysis of eugenol in extract of syzygium Aromaticum and pharmaceutical formulation. Statistical treatment of the observed voltammetic data revealed high accuracy and good precision of determination. The work has been supplemented by FTIR screening of the sample; eugenol exhibits absorption bands at about 3530 cm-1, 1510 cm-1, 1265 cm-1, 1035 cm-1 and 910 cm-1. The percentage of eugenol in pharmaceutical formulation was determined by the developed method, which is in close agreement with that reported by the manufacturers.