The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G1 interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15+ promoter that we call a PCB (pombe cell cycle box), which confers M-G1-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12+, cdc19+, fin1+, sid2+, ppb1+, mid1+/dmf1+ and plo1+, which we find to be transcribed at M-G1. A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
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Anderson, M., Ng, S. S., Marchesi, V., MacIver, F. H., Stevens, F. E., Riddell, T., … McInerny, C. J. (2002). Plo1+ regulates gene transcription at the M-G1 interval during the fission yeast mitotic cell cycle. EMBO Journal, 21(21), 5745–5755. https://doi.org/10.1093/emboj/cdf564