Estimation of malondialdehyde and catalase in pregnant & non-prenant women

Abstract

Backgrounds: Pregnancy is a period of increased demands of metabolism with changes in physiology of pregnant women and the requirements of oxygen increase in both mother and fetus. In Pregnancy due to increase demand of oxygen lead to development of oxidative stress within maternal circulation as well as in the placenta. The objective was to estimate Oxidative stress and antioxidant level in pregnancy as compare to non pregnant women. A total sample of 74 cases was taken of which 37 non pregnant and 37 pregnant women were within the age group of 18-40 years. 3 ml of venous blood was collected using a disposal syringe under aseptic condition in a, EDTA vial. 2.0 ml blood (plasma) was used for estimation of MDA and 1.0 ml blood for estimation of CATALASE. Results: The assessment of oxidants and antioxidants reveal highly significant increase (p<0.0001) in the levels of Malondialdehyde in Pregnant women when compared to non-Pregnant women and highly significant decrease (p<0. 0001) was found in the levels of Catalase in Pregnant women when compared to non Pregnant women. Karl Pearson's Coefficient Correlation, showed negative correlation between MDA and Catalase. Conclusion: The present study revealed that MDA level is significantly increased antioxidant and Catalase is significantly decreased in pregnant women as compared to non-pregnant women. Hence, the hypothesis that oxidative stress occured in pregnancy was strongly supported due to increased concentration of Malonadialdehyde and decreased concentration of Catalase in pregnant women.