The Major Phyto-Compounds Heptasiloxane, hexadecamethyl- and 1,1-Dimethylethyl 3-Phenyl-2-Propenoate Derived from Indigofera tinctoria Medicinal Flora Tested Against Various Target Medical and Agronomic Pests

Abstract

Objectives: To determine the phytochemical screening and major phyto-compounds from Indigofera tinctoria leaf methanol extract as well as ovicidal and larvicidal toxicity towards medical and agronomic pests, Aedes aegypti, Culex quinquefasciatus, Spodoptera litura and Helicoverpa armigera. Methods: In this study, the phytochemical screening was done by prescribed method, finding of phyto-constituents were made by GC-MS analysis, ovicidal and larvicidal toxicity of selected pests were recorded after 24 hrs. post treatment at various concentrations. The mortality was assessed by using probit analysis to calculate LC 50 /LC 90. Findings: By the phytochemical screening, the more numbers of phytochemicals were obtained from methanol extract as well as GC-MS analysis displayed sum of 10 phyto-compounds gained 100% besides two phyto-compounds were major constituents Heptasiloxane, hexadecamethyl-and 1,1-Dimethylethyl 3-Phenyl-2-Propenoate. The phyto-products of I. tinctoria were produced maximum eggs toxicity around 100% at higher concentration. Similarly, I. tinctoria leaf methanol extract borne major phyto-compounds major constituents Heptasiloxane, hexadecamethyl-and 1,1-Dimethylethyl 3-Phenyl-2-Propenoate were tested 3 rd instar larvae of selected medicinal and agronomic pests with their LC 50 / LC 90 value were 10.