Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead

Abstract

[Abstract] The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34 + cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34 + cells in blood is 0.1% of mononuclear cells. Materials and Reagents