IMMUNOHISTOCHEMICAL DETECTION OF L CELLS IN GASTROINTESTINAL TRACT MUCOSA OF PATIENTS AFTER SURGICAL TREATMENT FOR CONTROL OF TYPE 2 DIABETES MELLITUS

Abstract

RESUMOABSTRACT-–Racional:BACKGROUND:O tratamentoType 2 diabetesde escolhamellitus (T2DM)para pacientesis a diseasecomof globalhipertensãoimpact thatportalhas esquistossomóticaled an increase incomcomorbiditiessangramentoand mortalityde varizesin severalé a countries.desconexãoImmunoexpressionázigo-portal ofmaisthe incretin hormones such as glucagon-like peptide-1 (GLP-1) and peptide YY (3–36) (PYY3–36) can be esplenectomia used as a scorer (DAPE) in the gastrointestinal associada à terapia tract to endoscópica. analyze L-cell activity Porém, in estudos response mostram to T2DM aumento treatment. doOBJECTIVE:calibre dasThisvarizesstudyemaimedalgunstopacientesinvestigatedurantethe presence,o seguimentolocation,emandlongosecretionprazo.ofObjetivo:L cells in Avaliarthe smallo impactointestinedaof DAPEpatientse tratamentoundergoing endoscópicothe form of bariatricpós-operatóriosurgery denominatedcomportamentoadaptive dasgastroenteromentectomyvarizes esofágicas e recidivawith partialhemorrágica,bipartition.de pacientesMETHODS:esquistossomóticos.ImmunohistochemicalMétodos:assays, Foramquantitativeestudadosreal-time36polymerasepacientes chaincom reactionseguimento(qPCR),superiorand Westerna cincoblotanos,analysisdistribuídoswere performedem on samples of intestinal mucosa from patients with T2DM in both the preoperative and postoperative dois periods. grupos: RESULTS: queda All da results pressão were portal consistent abaixo and de indicated 30% e basal acima expression de 30% and comparados secretion of com GLP-1 o calibreand PYYdas3–36 varizesincretinsesofágicasby L cells. Anogreaterpós-operatóriodensity of cellsprecocewas demonstratede tardio alémin thedo índicemost distalde recidivaportions hemorrágica.of the small intestine.ResultadosNo significant difference was found between GLP-1 and PYY3–36 expression esofágicaslevels in the que,preoperativedurante oandseguimentopostoperativeaumentaramperiods becausede calibreof prolongede foramfastingcontroladasduring whichcom the samples were collected. CONCLUSION: The greater number of L cells in activity implies better peptide signaling, response, and functioning of the neuroendocrine system.