The GAR domain of GAS2L3 mediates binding to the chromosomal passenger complex and is required for localization of GAS2L3 to the constriction zone during abscission

Abstract

GAS2L3 is a recently identified tubulin- and actin-binding protein that regulates cytokinesis and abscission. In this study we show that GAS2L3 interacts with the chromosomal passenger complex (CPC), which plays key roles in mitosis and cytokinesis. Biochemical assays show that GAS2L3 directly interacts with the C-terminus of borealin and the N-terminus of survivin. We find that the interaction between these two CPC subunits and GAS2L3 is mediated by the conserved GAR domain of GAS2L3. We further show that the GAR domain of GAS2L3 is required for localization of GAS2L3 to the constriction zone. Taken together these data suggest that GAS2L3 is a downstream effector of the CPC during cytokinetic abscission. GAS2L3 is a recently identified tubulin- and actin-binding protein that regulates cytokinesis. Here, we show that GAS2L3 interacts via its conserved GAR domain with the chromosomal passenger complex (CPC) and we report that the GAR domain is required for localization of GAS2L3 to the constriction zone. This suggests that GAS2L3 is a downstream effector of the CPC during cytokinetic abscission. © 2014 FEBS.