Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein

Abstract

Background: Procoat protein mutants require the Sec translocase in addition to YidC. Results: Subtle changes in the polarity of the translocated region alter the mode of insertion. Conclusion: The translocase requirement increases as the energy transfer expense of the translocated region increases. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.