Inactivation of penaeid rod-shaped DNA virus (PRDV), the causative agent of penaid acute viremia (PAV), by some chemical and physical treatments

Abstract

Penaeid rod-shaped DNA viruses (PRDV) in tissue homogenates of the naturally infected shrimp were subjected to various chemical and physical treatments, and inactivation of the virus was examined by infection experiment. The infection was examined by the appearance of the virus-infected nuclei in stomach cuticular epidermis. Disinfectants: The virus was inactivated by 1 min contact with 0.62 mg/l of oxidant or 30% of ethyl alcohol, by 10 min contact with 1 mg/l of sodium hypochlorite, 2.5 mg/l of povidone iodine, 5 g/l of formalin or 25 mg/l of trimethylammoniummethylene chloride, and by 60 min contact with 5,000 mg/l of sodium carbonate peroxyhydrate at 25°C. NaCl: The virus was inactivated with 25% NaCl solution within 24 h. Chloroform: The virus was inactivated with chloroform within 15 min. Heat: The virus was inactivated within 60 min at 50°C, 1 min at 60°C and 0.2 min at 70°C, respectively. Desciccation: The virus absorbed in a filter paper was inactivated within 3 h desciccation at about 26°C. UV. irradiation: PRDV was inactivated with U. V. irradiation of 1 × 104 μW · sec/cm2.