Quantitative fluorescence image analysis in bladder cancer screening

Abstract

Quantitative fluorescence image analysis (QFJA) cytology combines image analysis to measure DNA with visual cytology for bladder cancer detection. QFIA sensitivity is 76% to 81% and 96% to 100% for low- and high-grade tumors respectively, with 94% specificity in asymptomatic controls. QFIA screening of 504 persons within a β-naphthylamine exposed cohort found DNA hyperploidy correlated with the duration of caroinogenio exposure and smoking history; marker prevalence was 83% for exposed workers who smoked and 2% for nonexposed nonsmokers. In prospective animal studies, QFIA was useful in monitoring caroinogenesis and ohemoprevention with retinoids. Current QFIA research focuses on measurements of oncogenes, growth factors and their receptors, cytoskeleton, and tumor-associated antigens to improve sensitivity and specificity to low-grade tumors and to identify premalignant conditions. Profiles of biochemical and immunological markers on single cells may further assist in the study of high-risk cohorts and individual risk assessment. © by American College of Occupational Medicine.