μGISAXS and protein nanotemplate crystallization: Methods and instrumentation

Abstract

Microbeam grazing-incidence small-angle X-ray scattering (μGISAXS) has been used and the technique has been improved in order to investigate protein nucleation and crystal growth, assisted by a protein nanotemplate. The aim is to understand the protein nanotemplate method in detail, as this method has been proved capable of accelerating and increasing crystal size and quality as well as inducing crystallization of proteins that are not crystallizable by classical methods. The nanotemplate experimental setup was used for drops containing growing lysozyme crystals at three different stages of growth. © 2005 International Union of Crystallography Printed in Great Britain - all rights reserved.