Association of bovine tumor necrosis factor alpha gene polymorphism with mastitis in Nili Ravi buffaloes

Abstract

The current research was designed to find out polymorphic changes in complete gene sequence of tumor necrosis factor alpha (TNF-α), which is involved in inducing mastitis in Nili Ravi buffaloes. Total 30 blood samples were collected having clinical (n=10), subclinical (n=10) mastitis and normal (n=10) animals. DNA was extracted, TNF-α gene was amplified and sequenced. Homology analysis of TNF-α gene sequences using Finch TV tool revealed total of eleven SNP’s in subclinical mastitic buffaloes and twelve in clinical mastitic buffaloes, including insertion, heterozygosity, transitional and some silent mutations; in intronic region. In subclinical mastitis samples, insertion of “G” at the position 279, transition mutation of (A/G) at position 1022, Heterozygosity of (A/G) at 1023 cite, transition mutation of (A/G) at position 1129, deletion of “C” at position 1741 and transition of (A/G) were found at cite 2091 of this gene, respectively. In the clinical mastitis samples, there was an insertion of “G” at the position 279, transition mutation of (A/G) at position 1022 and Heterozygosity of (A/G) at 1023 cite were found. All these SNPs were not found in sequences of normal animals. The current genome association study showed the potential correlation between these significant polymorphisms and incidence of clinical and subclinical mastitis in Nili Ravi buffaloes.