Commercial growth regulator has adverse effect over soybean seedlings under different cadmium levels

Abstract

Aim of study: Soils contaminated by heavy metals, such as cadmium, may reduce plant development. Exogenous application of plant growth regulators (PGR), are used for optimizing the crops production in stressful environments. The aim of this study was to evaluate the effects of Cd concentrations on the development of soybean seedlings under exogenous application of a commercial PGR. Area of study: Presidente Prudente, São Paulo, Brazil. Material and methods: Soybean seeds were pre-treated in distilled water (control treatment) and in solution with plant growth regulator (PGR treatment) and then germinated with distillated water. The germinated seeds were transferred to different levels of Cd (0, 100, 500 and 900 mg L-1). Main results: Cd exposure at increasing concentrations, decreased root development, (area, length and volume of roots) and activity of enzymatic antioxidants (SOD, CAT and APX) and enhanced MDA. These responses were accentuated by the PGR ex-position. The root morphology and activity of antioxidant enzymes presented “hormesis” responses until 500 mg L-1 of Cd, and the proline content may have played a fundamental role in the maintenance of metabolic activities and biomass. Research highlights: The results indicate that the use of PGR intensified the toxicity responses caused by exposure to increased Cd level. In addition, stress indicators such as MDA content and antioxidant activity in different organs (root and shoot) of soybean seedlings, responded differently according with the use of PGR under exposure of Cd.