HPTLC analysis of stem bark extracts of Terminalia Chebula retz. For Alkaloid profile

Abstract

Terminalia chebula Retz. commonly known as Haritaki is a deciduous tree having extraordinary powers of healing with a wide spectrum of biological activity. The aim of the present study was to check the presence of phytochemical constituent alkaloid, using HPTLC analysis. The stem bark extracts of Terminalia chebula was prepared using different solvents like methanol, chloroform and petroleum ether. The extracts have been subjected to HPTLC analysis for alkaloid profile. Colchicine was used as standard. The presence of alkaloid in all the three solvent extract samples were confirmed based on the colour zones obtained. The presence of alkaloid was indicated by bright orange and brown coloured zones under daylight mode. High performance thin-layer chromatography analysis is a powerful technique and thereby significantly increased precision and high throughput Hence this profile analysis would pave the way for the future phytoconstituents detection and herbal formulation.