Background: To simplify clinical scale lymphocyte expansions, we investigated the use of the WAVE ®, a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes.Methods: We have developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant numbers of cells for our adoptive cell transfer clinical protocols.Results: TIL and genetically modified PBL were rapidly expanded to clinically relevant scales in both static bags and the WAVE bioreactor. Both bioreactors produced comparable numbers of cells; however the cultures generated in the WAVE bioreactor had a higher percentage of CD4+ cells and had a less activated phenotype.Conclusions: The WAVE bioreactor simplifies the process of rapidly expanding tumor reactive lymphocytes under GMP conditions, and provides an alternate approach to cell generation for ACT protocols. © 2012 Somerville et al; licensee BioMed Central Ltd.
CITATION STYLE
Somerville, R. P. T., Devillier, L., Parkhurst, M. R., Rosenberg, S. A., & Dudley, M. E. (2012). Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ®bioreactor. Journal of Translational Medicine, 10(1). https://doi.org/10.1186/1479-5876-10-69
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