Detection of human pathogenic gram-negative bacteria from ornamental goldfish according to gene sequence alignment

Abstract

Identification of bacterial infections involved in ornamental fishes has a great importance with considering the adverse effects on the aquatic health as well as the possibility of common diseases between human and fish. In the present study pathogenic bacteria were isolated and purified from skin, gill, kidney and brain of symptomatic goldiish samples from aquaculture ponds in Iran on the enriched culture media. The isolated bacteria have been first identified according to the morphological and biochemical characteristics. Molecular detection was done based on the amplification of specific genome fragments and sequencing of the amplified nucleotides. The isolated bacteria detected as Pseudomonas mendosina and Aeromonas spp. including Aeromonas hydrophila (the most sequence similarity), Aeromonas veronii, Aeromonas sobria and Aeromonas salmonicida (partial similarity in the genome sequence). The results of present study showed that gold fishes which are among the most favorable ornamental fishes, can be carriers of human pathogens from Pseudomonas and Aeromonas genera and this fact is necessary to considered in aquaculture healthcare systems. © 2014 Asian Network for Scientific Information.