Analysis by RP-HPLC of mangiferin component correlation between medicinal loranthus and their mango host trees

Abstract

This study proposes a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of mangiferin in medicinal loranthus shrubs and their mango or non-mango host trees. Mangiferin in samples was extracted with a solution of 40% methanol. Analytical determination was conducted by RP-HPLC with ultraviolet detection at 258 nm. Chromatographic separation was achieved on an Inertsil ODS-SP column (250 × 4.6 mm, 5 μm) by isocratic elution with methanol-0.1% aqueous phosphoric acid (31:69, v/v). Mangiferin contents were 5.04 to 18.95 mg/g in mango trees and 0.44 to 3.72 mg/g in medicinal loranthus parasitized on mango host trees. Mangiferin could not be detected in non-mango trees and their loranthus shrubs. This study indicated that host trees could affect the quality of medicinal loranthus by transporting host-inherent components into loranthus. © 2012 Crown copyright.