A new dressing material in diabetic wounds: Wound healing activity of oleuropein-rich olive leaf extract in diabetic rats

Abstract

Introduction: The objective of this study was to evaluate a dressing material on ischemic wound model in diabetic rats. Study was conducted during the months of June 2012-March 2013 at Ege University in Izmir, Turkey.Materials and Methods: Spraque Dawley rats weighing 200-250 g obtained from an experimental animal production center (Saki YeniliAnkara,Turkey) were used in this study. Animals were randomly assigned to diabetic (n= 20) and nondiabetic (n= 20) groups. After diabetes induction and wound creation, animals within each group were assigned to two wound dressing groups by a second randomization. The study was carried out on these four groups. After diabetes induction and wound creation, animals within each group (n= 10) were assigned to olive leaf extract (OLE) wound dressing and normal saline (sodium chloride, 0.9% NaCl) wound dressing groups by a second randomization. 0.9% NaCl and OLE dressing was applied to wounds once a day by the researcher. The areas of wounds were measured by Walker Formula. OLE wound dressing healed wounds faster and earlier than classic wound dressing (p< 0.05).Results: In the diabetic group; wounds closure time was found to be 24.80 ± 1.48 in OLE wound dressing and 28.00 ± 2.31 days in classical wound dressing.Conclusion: As a result in terms of success ratios, OLE wound dressing for wound healing in diabetic and non-diabetic wounds has been determined to be more effective in comparison with classic wound dressing method.