Immunological reactivity of blood from healthy humans to the rAls3p-N vaccine protein

Abstract

We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and im-munoglobulin E (median, 1:128) titers to rAls3p-N by en-zyme-linked immunosorbent assay. rAls3p-N stimulated in-terferon α (IFN- α) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-Y, IL-17, and IL-1O by cyto- metric bead array. Donors reacted to diverse immunodom-inant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials. © 2009 by the Infectious Diseases Society of America. All rights reserved.