Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation

Abstract

Identification of modification sites for ubiquitin/Ubls is critical for elucidating the regulated processes. Lobato-Gil et al. report the proteome-wide identification of NEDD8 sites, which defines distinct NEDD8 proteomes for canonical and atypical NEDDylation and formation of diverse homo- and hybrid NEDD8 polymers. Proteotoxic-stress-induced NEDD8-SUMO-2 chains accumulate in nucleolus-related inclusions.