Identification of basidiomycetes in decayed wood by a method using non-specific amplification of DNA

Abstract

A combination of non-specific amplification of DNA by Phi DNA polymerase and polymerase chain reactionPCRusing the specific primers for the internal transcribed spacerITSregion of ribosomal DNA rDNA enables us sensitive detection of basidiomycetes in decayed wood and identification of their species by BLAST analysis of DNA sequence on databaseThis method was applied for detection and identification of basidiomycetes in wood blockswhich had been settled in bait stations around a site of house As the results DNA sequences of ITS region were obtained for samples from bait stationsThe corresponding species of basidiomycete were identified for sampleswhich distributes species in generasuggesting a high diversity of fungal flora depending on micro-environment within a site of house Key wordsbasidiomyceteswood rotting fungiPhi DNA polymeraseInternal Transcribed Spacer regionPCR Phi DNA DNA DNArDNAInter-nal Transcribed SpacerITSPCR DNA BLAST ITS