Changes in anopheles funestus biting behavior following universal coverage of long-lasting insecticidal nets in benin

Abstract

Background. Behavioral modification of malaria vectors in response to vector control methods is of great concern. We investigated whether full coverage of long-lasting insecticide-treated mosquito nets (LLINs) may induce a switch in biting behavior in Anopheles funestus, a major malaria vector in Africa.Methods.Human-landing collections were conducted indoor and outdoor in 2 villages (Lokohouè and Tokoli) in Benin before and 1 year and 3 years after implementation of universal LLIN coverage. Proportion of outdoor biting (POB) and median catching times (MCT) were compared. The resistance of A. funestus to deltamethrin was monitored using bioassays.Results.MCT of A. funestus switched from 2 am in Lokohoué and 3 am in Tokoli to 5 am after 3 years (Mann-Whitney U test, P