Nitrogen compounds extracted by electroultrafiltration (EUF) or CaCl2 solution and their relationships to nitrogen mineralization in soils

Abstract

The objective of the investigation was to identify the most important organic N-containing fractions extracted from soils by electroultrafiltration (EUF) or a CaCl2 solution, respectively, and their importance for nitrogen mineralization. The investigation comprised 19 agricultural and one forest top soil. Net N mineralization was tested in Mitscherlich pot experiments with three treatments: (1) fallow soil without N fertilizer, (2) soil cultivated with rye grass without N fertilizer, (3) soil cultivated with rye grass with N fertilizer. The highest proportion of N in the extracts was the amino N fraction (amino acids + peptides) amounting to approximately 60 % of the total N extracted by CaCl2 and to about 40 % of the total N extracted by EUF. The proportion of amino sugars from total N extracted was in average 10 % for the CaCl2 and 5.2 % for the EUF extracts. The proportion of heterocyclic N bases derived from nucleic acids amounted in average to 4.8 % and 3.6 % for the CaCl2 and EUF extract, respectively. Amino N (amino acids + peptides) were correlated best with net N mineralization (EUF, r = 0.81***, CaCl2, r = 0.86***). The correlation between amino sugars and net N mineralization was r = 0.55* for the EUF extract and r = 0.49* for the CaCl2 extract. The heterocyclic N bases did not correlate with net N mineralization. Correlations between Norg extracted by CaCl2 versus net N mineralization were higher than those obtained by the EUF extract. Net N mineralization was about four times higher in the fallow soils than in the treatment with grass and no N fertilizer. In the treatment with grass + N fertilizer on average no net N mineralization occurred, moreover there was a tendency of N immobilization. It is assumend that in the treatments with grass cultivation, organic C released by roots stimulated the assimilation of mineral N and amino acids by soil microorganisms resulting in a low net N mineralization. Net N mineralization led to a highly significant depletion in the Norg pools and particularly in the amino N and amino sugar pools in the treatment with grass and without N fertilizer. This depletion was particularly evident in the CaCl2 extracts. The results justify the conclusion that the Norg obtained with both extraction methods originates from a dynamic N pool into which N flows in and out. The amino N extractable with EUF or CaCl2 is a reliable indicator for the net N mineralization potential of soils. © WILEY-VCH Verlag GmbH 1999.