Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

Abstract

By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as "RDV-size fraction (RDV-SF)". Using RDV-SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells. © 2010 Watanabe S, et al.